• Is it possible to use the "Sample Blank" bottle of Bilirrubina total AA for Bilirrubina Directa AA?
NO
• In patients with HEPATIC disorders a low sensitivity of Direct Bilirubin with respect to Total Bilirubin is observed, thus obtaining differing values.
In order to provide for these situations, the programming of bilirubin determinations has been modified. It will be sent on customer's demand. • Reagent 2 became green colored. Can it still be used?
Yes, since it doesn't affect its functionality, which can be tested through the processing of serum controls (Standatrol SE 2 Levels). • Lack of reproducibility in determinations of ASO, PCR, and FR.
It may be due to a lack of homogeneity in the latex reagent. Remove the latex reagent from the autoanalyzer, shake it and perform the determinations again.
• No reproducibility is observed in the determination.
To achieve a high accuracy for the intended use of this reagent, the following is recommended:
- Work following good laboratory practices, which in the case of phosphorus recommend maximizing the cleaning of all work materials:
- Wash carefully the reagent bottles with HCl 5% and rinse them with plenty of demineralized water. Before decanting the reagent into the bottle, rinse the bottle with a small proportion of the reagent and discard it.
- If pediatric sample cups are to be used, make sure they are new.
- Use new cuvettes or unique ones for this determination.
- Check previously the proper functioning of the washing system.
- Calibration - recommended frequency: weekly (or after a change of reagent lot).
- Once the calibration has been verified, process the phosphorus determinations separately.
• I am using the Cal A Plus and after calibrating glucose, urea, and triglycerides I get quality control values raised by 30% approximately; likewise, patients show higher results than those usually found for these metabolites.
These high values are not showed in other determinations which I haven't calibrated.
What could it be?
Apparently, the problem has to do with the calibration since other determinations show coherent values and it is unlikely that different reagents start overestimating at the same time.
We suggest checking the way you prepare the Cal A plus. It has to be rehydrated with only 3 ml of tonic (included in the equipment), and it is necessary to wait for 30 minutes before using it.
• I am starting to use the HDL Colesterol FT kit, HDL Colesterol Reactivo Precipitante and LDL Colesterol Reactivo Precipitante and I have doubts about how to calibrate them and with what.
You can use the Cal A plus or the watery standard which is supplied with the cholesterol reagent.
In the case of the first one, no previous treatment is required; it should be used pure and the values are those outlined in the enclosed packaging insert. If the watery standard is used, the concentrations must be the ones outlined in the packaging insert, which are 0.762 g/l for HDL Colesterol FT, 0.457 g/l for HDL Colesterol Reactivo Precipitante, and 0.624 g/l for LDL Colesterol Reactivo Precipitante; in this case, no previous treatment is required.
In both cases, the proportion sample-reagent indicated in the packaging insert must be followed.
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