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Frecuently Asked Questions




1. Bilirrubina Total AA y Bilirrubina Directa AA



    • Is it possible to use the "Sample Blank" bottle of Bilirrubina total AA for Bilirrubina Directa AA?

    NO

    • In patients with HEPATIC disorders a low sensitivity of Direct Bilirubin with respect to Total Bilirubin is observed, thus obtaining differing values.

    In order to provide for these situations, the programming of bilirubin determinations has been modified. It will be sent on customer's demand.

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2. ALP 405 AA líquida

    • Reagent 2 became green colored. Can it still be used?

    Yes, since it doesn't affect its functionality, which can be tested through the processing of serum controls (Standatrol SE 2 Levels).

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3. Creatinina Cinética AA líquida

          · Reagent does not work properly. The sample results differ from those for the previous reagent.

          The proportion of A & B reagent is different to the previous one. The programmings in all autoanalyzers have been modified.


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4. Proteínas – Inmunoturbidimetría – Látices

    · Lack of reproducibility in determinations of ASO, PCR, and FR.

    It may be due to a lack of homogeneity in the latex reagent.  Remove the latex reagent from the autoanalyzer, shake it and perform the determinations again.


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5. FÓSFORO UV en Metrolab 2300 Plus


    · No reproducibility is observed in the determination.

    To achieve a high accuracy for the intended use of this reagent, the following is recommended:

    • Work following good laboratory practices, which in the case of phosphorus recommend maximizing the cleaning of all work materials:
    o Wash carefully the reagent bottles with HCl 5% and rinse them with plenty of demineralized water. Before decanting the reagent into the bottle, rinse the bottle with a small proportion of the reagent and discard it.
    o If pediatric sample cups are to be used, make sure they are new.
    • Use new cuvettes or unique ones for this determination.
    • Check previously the proper functioning of the washing system.
    • Calibration - recommended frequency: weekly (or after a change of reagent lot).
    • Once the calibration has been verified, process the phosphorus determinations separately.


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6. Calibrador A Plus

    · I am using the Cal A Plus and after calibrating glucose, urea, and triglycerides I get quality control values raised by 30% approximately; likewise, patients show higher results than those usually found for these metabolites.
    These high values are not showed in other determinations which I haven't calibrated.
    What could it be?


    Apparently, the problem has to do with the calibration since other determinations show coherent values and it is unlikely that different reagents start overestimating at the same time.
    We suggest checking the way you prepare the Cal A plus. It has to be rehydrated with only 3 ml of tonic (included in the equipment), and it is necessary to wait for 30 minutes before using it.


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7. HDL Colesterol FT, HDL Colesterol Reactivo Precipitante y LDL Colesterol Reactivo Precipitante

    I am starting to use the HDL Colesterol FT kit, HDL Colesterol Reactivo Precipitante and LDL Colesterol Reactivo Precipitante and I have doubts about how to calibrate them and with what.

    You can use the Cal A plus or the watery standard which is supplied with the cholesterol reagent.
    In the case of the first one, no previous treatment is required; it should be used pure and the values are those outlined in the enclosed packaging insert. If the watery standard is used, the concentrations must be the ones outlined in the packaging insert, which are 0.762 g/l for HDL Colesterol FT, 0.457 g/l for HDL Colesterol Reactivo Precipitante, and 0.624 g/l for LDL Colesterol Reactivo Precipitante; in this case, no previous treatment is required.
    In both cases, the proportion sample-reagent indicated in the packaging insert must be followed.

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8. Metrolab 2300 Plus

    • In relation to the programming of the "Samples and Reagents Tray", is it possible to place any reagent in a Kahn tube inside a bottle?

    No. The volume of reagent used by the method must be less than 250 ul.

    • How is it indicated in the analyzer that the reagent has been placed in a tube, a bottle of 20 ml, 30 ml, or 50 ml?

    · If the reagent has been placed in a 50 ml bottle, you should only check "full size".
    · If the reagent has been placed in a 30 ml or 20 ml bottle, you should check "split" and "full size".
    · If the reagent has been placed in a tube inside a 50 ml bottle, you should uncheck "full size".
    · If the reagent has been placed in a tube inside a 30 ml bottle, you should uncheck "full size" and check only "split".
    · If the reagent has been placed in a tube inside a 20 ml bottle, you should uncheck "full size".

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1. Coagulation: How can I check my coagulation reagents are working properly?


    Performing determinations with Normal and Pathological Control Plasma, and checking that the values obtained are within the range specified in the packaging insert.

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2. Soluplastin: Why are Prothrombin times in "normal" individuals longer than usual?

    Soluplastin currently presents sensitivity values (ISI) that range between 1.25 – 1.35. This increase of the sensitivity makes "normal" values (usually 10-12 sec.) to be longer, between 13-15 sec.  This phenomenon becomes more noticeable when the technique is performed manually.

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3. Soluplastin: Why isn't the prothrombin rate table showed in the yellow sheet enclose?

    Because those values belong to a curve performed in our laboratory. These conditions may not be the same in all institutions. It is a good praxis rule to perform a "curve of times" vs. "prothrombin rate" on each laboratory and particular situation (operators, techniques, and consumables).

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4. Fibrintimer-2/ Soluplastin: What should I consider for the correct development of Soluplastin in Fibrintimer-2?

    1. Soluplastin: The reagent must be shaken constantly (being a lipid suspension). This can be achieved by placing the Soluplastin container in the 1st hole on the left (which is shaken) with the shaker magnet "ad Hoc" inside the reagent.
    2. It is necessary to pre-incubate 50 ul of plasma for 60 sec. and then trigger off the reaction with the 100ul Soluplastin aggregate.
    3. Follow the work order indicated on the display for the determination.


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Fibrintimer-2/ APTT: What should I consider for the correct development of APTT in Fibrintimer-2?

    1. APPTest ellágico is the preferred reagent for any coagulometer since it is a liquid solution. Having diatomaceous earth as activator, APPTest is a suspension with a cloudier background, thus making visualization difficult.
    2. APTTest ellágico shouldn't be incubated at 37º; only the calcium chloride should be incubated (reaction trigger).
    3. It is necessary to pre-incubate 50ul plasma + 50ul APPTest ellágico for 120 sec. and then trigger off the reaction with the 50ul calcium chloride aggregate. 4. Follow the work order indicated on the display for the determination.


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6.Reaclon Anti-A: Reagent composition

    Murine monoclonal antibody (IgM). BIRMA-1 clone

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7.Reaclon Anti-AB: Reagent composition

          Mixture of murine monoclonal antibodies of the IgM class (Blend), BIRMA-1 clones / ES-4 / ES-15.

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8.Reaclon Anti-B: Reagent composition

    Murine monoclonal antibody of the IgM class, LB-2 clone.

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9. Reaclon Anti-D: Reagent composition

    Mixture of human monoclonal antibodies IgM/IgG (Blend) belonging to TH-28 clones (IgM) and MS-26 (IgG).

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10.Reaclon Anti-D: Does it detect the DVI variant?

          This reagent is able to detect the DVI antigen through the indirect antiglobulin method (Coombs).

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11. HIV Test ELISA: What generation does it belong to?

          HIV Test ELISA belongs to the second generation of reagents (Anti-Immunoglobulin conjugate).

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12. Hepatitis B (anti-HBc) ELISA: Unlike the rest of ELISA in the line, why are negative controls colored and positive ones colorless?

    Because it is a competitive method.

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Technical Service FAQs

General FAQs


1- Can Technical Service engineers give me an answer to reagents adaptation problems?

No. Chemical/biochemical advisors must be consulted about reagents adaptation problems.


2- Can I call Technical Service to repair instruments other than the ones commercialized by Wiener lab.?

Engineers who directly depend on Wiener lab or any of its associate companies DO NOT repair instruments other than the ones commercialized by Wiener lab. It may happen that in your region Technical Service is carried out by personnel hired by the company. In such case, it is possible that they do this kind of repairs.


3- Do Technical Service engineers provide training as regards the use of instruments?

NO. That is what Chemical/Biochemical Technical Consultants do.


4- Can TS give information regarding prices of consumables, accessories, and reagents?

They do not usually have that information, which is the sales personnel job.


5- Is it possible to have in-home service for a spectrophotometer? Which is the delivery time?

No, the equipment must be taken to the nearest Technical Service. The maximum delivery time is 15 days.


6- What kind of services do you provide for autoanalyzers and how can I request them?

We provide preventive and corrective maintenance for particular events, which have to be previously budgeted for; we also offer a technical service subscription that covers your equipment against failures which may require corrective actions, and includes scheduled preventive visits.




FAQs about Wiener lab. Selectra I/II

1 Problem: It gives a water error.
Solution: Check the level of water in the can, raise the can to the monitor level and remove the water filter from the pickup tube.

2 Problem: No sample is detected.
Solution: Check that the needle is not wet. Check that the detection wire isn't broken or cut.

3 Problem: Leak in the syringe.
Solution: Check that the leak is actually in the syringe and not in the pipettor valve. If it is in the syringe, then change the tip.



FAQs about Wiener lab. Metrolab 2300 plus

1 Problem: Software does not open.
Solution: Recover BU file from the previous day.

2 Problem: It doesn't wash properly
Solution: Check the condition of the Teflon dryer and clean the lateral filters (right side).



FAQs about Wiener lab. Counter 19:

1 Problem: Wrong blanks reading.
Solution: Perform the cuvette washings and respective openings.

Next Events
  EEUU

July, 27th to 29th

2010 AACC/CSCC Annual Meeting and Clinical Lab Expo

Wiener lab., booth n. 1406

Anaheim, California
USA

More information:
http://www.aacc.org/events
  Alemania/Germany

November 17th to 20th, 2010

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Düsseldorf,
Germany

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Not all the products are commercialized in every country. Please contact your local agent/representative.