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Making the future possible
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FAQ

• Is it possible to use the "Sample Blank" bottle of Bilirrubina total AA for Bilirrubina Directa AA?

NO

• In patients with HEPATIC disorders a low sensitivity of Direct Bilirubin with respect to Total Bilirubin is observed, thus obtaining differing values.

In order to provide for these situations, the programming of bilirubin determinations has been modified. It will be sent on customer's demand.

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• Reagent 2 became green colored. Can it still be used?

Yes, since it doesn't affect its functionality, which can be tested through the processing of serum controls (Standatrol SE 2 Levels).

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Lack of reproducibility in determinations of ASO, PCR, and FR.

It may be due to a lack of homogeneity in the latex reagent. Remove the latex reagent from the autoanalyzer, shake it and perform the determinations again.


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No reproducibility is observed in the determination.

To achieve a high accuracy for the intended use of this reagent, the following is recommended:

  • Work following good laboratory practices, which in the case of phosphorus recommend maximizing the cleaning of all work materials:

    • Wash carefully the reagent bottles with HCl 5% and rinse them with plenty of demineralized water. Before decanting the reagent into the bottle, rinse the bottle with a small proportion of the reagent and discard it.
    • If pediatric sample cups are to be used, make sure they are new.
  • Use new cuvettes or unique ones for this determination.
  • Check previously the proper functioning of the washing system.
  • Calibration - recommended frequency: weekly (or after a change of reagent lot).
  • Once the calibration has been verified, process the phosphorus determinations separately.

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• I am using the Cal A Plus and after calibrating glucose, urea, and triglycerides I get quality control values raised by 30% approximately; likewise, patients show higher results than those usually found for these metabolites.
These high values are not showed in other determinations which I haven't calibrated.
What could it be?

Apparently, the problem has to do with the calibration since other determinations show coherent values and it is unlikely that different reagents start overestimating at the same time.
We suggest checking the way you prepare the Cal A plus. It has to be rehydrated with only 3 ml of tonic (included in the equipment), and it is necessary to wait for 30 minutes before using it.

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I am starting to use the HDL Colesterol FT kit, HDL Colesterol Reactivo Precipitante and LDL Colesterol Reactivo Precipitante and I have doubts about how to calibrate them and with what.

You can use the Cal A plus or the watery standard which is supplied with the cholesterol reagent.
In the case of the first one, no previous treatment is required; it should be used pure and the values are those outlined in the enclosed packaging insert. If the watery standard is used, the concentrations must be the ones outlined in the packaging insert, which are 0.762 g/l for HDL Colesterol FT, 0.457 g/l for HDL Colesterol Reactivo Precipitante, and 0.624 g/l for LDL Colesterol Reactivo Precipitante; in this case, no previous treatment is required.
In both cases, the proportion sample-reagent indicated in the packaging insert must be followed.

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​Because those values belong to a curve performed in our laboratory. These conditions may not be the same in all institutions. It is a good praxis rule to perform a "curve of times" vs. "prothrombin rate" on each laboratory and particular situation (operators, techniques, and consumables).

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​1. Soluplastin: The reagent must be shaken constantly (being a lipid suspension). This can be achieved by placing the Soluplastin container in the 1st hole on the left (which is shaken) with the shaker magnet "ad Hoc" inside the reagent.

2. It is necessary to pre-incubate 50 ul of plasma for 60 sec. and then trigger off the reaction with the 100ul Soluplastin aggregate. 

3. Follow the work order indicated on the display for the determination.

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​1. APPTest ellágico is the preferred reagent for any coagulometer since it is a liquid solution. Having diatomaceous earth as activator, APPTest is a suspension with a cloudier background, thus making visualization difficult.

2. APTTest ellágico shouldn't be incubated at 37º; only the calcium chloride should be incubated (reaction trigger).

3. It is necessary to pre-incubate 50ul plasma + 50ul APPTest ellágico for 120 sec. and then trigger off the reaction with the 50ul calcium chloride aggregate. 4. Follow the work order indicated on the display for the determination.

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​Murine monoclonal antibody (IgM). BIRMA-1 clone

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Mixture of murine monoclonal antibodies of the IgM class (Blend), BIRMA-1 clones / ES-4 / ES-15.

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​Murine monoclonal antibody of the IgM class, LB-2 clone.

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​Mixture of human monoclonal antibodies IgM/IgG (Blend) belonging to TH-28 clones (IgM) and MS-26 (IgG).

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​This reagent is able to detect the DVI antigen through the indirect antiglobulin method (Coombs).

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​No. Chemical/biochemical advisors must be consulted about reagents adaptation problems.

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​Engineers who directly depend on Wiener lab or any of its associate companies DO NOT repair instruments other than the ones commercialized by Wiener lab. It may happen that in your region Technical Service is carried out by personnel hired by the company. In such case, it is possible that they do this kind of repairs.

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​NO. That is what Chemical/Biochemical Technical Consultants do.

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​They do not usually have that information, which is the sales personnel job.

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​No, the equipment must be taken to the nearest Technical Service. The maximum delivery time is 15 days.

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​We provide preventive and corrective maintenance for particular events, which have to be previously budgeted for; we also offer a technical service subscription that covers your equipment against failures which may require corrective actions, and includes scheduled preventive visits.

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Problem: It gives a water error.
Solution: Check the level of water in the can, raise the can to the monitor level and remove the water filter from the pickup tube.

Problem: No sample is detected.
Solution: Check that the needle is not wet. Check that the detection wire isn't broken or cut.

Problem: Leak in the syringe.
Solution: Check that the leak is actually in the syringe and not in the pipettor valve. If it is in the syringe, then change the tip.

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Problem: Software does not open.
Solution: Recover BU file from the previous day.

Problem: It doesn't wash properly
Solution: Check the condition of the Teflon dryer and clean the lateral filters (right side).

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Problem: Wrong blanks reading.
Solution: Perform the cuvette washings and respective openings.

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Wiener Laboratorios SAIC - Date of last update: 03/07/2019 - Revision 002 - All rights reserved / All products are sold in all countries. Ask your agent / local distributor.
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